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KMID : 0616120120430010010
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Korean Journal of Pharmacognosy
2012 Volume.43 No. 1 p.10 ~ p.15
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Simultaneous Determination of Three Compounds in Ejung Tang by HPLC-DAD and LC-ESI-MS
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Lee Bo-Hyoung
Ma Jin-Yeul
Weon Jin-Bae
Yang Hye-Jin
Yun Bo-Ra
Ma Choong-Je
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Abstract
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An accurate and sensitive analysis method was established for simultaneous determination of three bioactive compounds (glycyrrhizin, 6-gingerol and ginsenoside Rg3) in the Ejung Tang with high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray ionization (ESI)-Mass spectrometry (MS). The optimizing chromatographic separations a were acquired by an C18 column (5 μm, 4.6 I.D × 250 mm, SHISHEDO) using gradient elution with water comprising 0.1% TFA(trifluoroacetic acid) and acetonitrile at a performing temperature of 35℃. Flow rate was 1.0 ml/min. A detection UV wavelength set at 205 nm and 250 nm. The three compounds were identified by electrospray ionization mass spectrometry. All calibration curves indicated great linear regression within test ranges (R²>0.9997). The established method provided acceptable precision and accuracy. The relative standard deviations (RSDs) of intra-day and inter-day were less than 2.00% and 3.00%, respectively. The recoveries were found to range from 94.49 to 101.10% for the three compounds analyzed. These results showed that this method was effective and reliable for quality control of Eiung-Tang.
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KEYWORD
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Ejung Tang, Simultaneous determination, HPLC-DAD, LC-ESI-MS, Validation
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